Generator

Part:BBa_K336053:Design

Designed by: Naoto Shimada   Group: iGEM10_Tokyo_Metropolitan   (2010-10-27)


Over expression system (T7 polymerase induced by lac promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

only design


Source

R0010:promoter (lacI regulated) B0034:RBS (Elowitz 1999) -- defines RBS efficiency K145001:T7 RNA polymerase B0015:double terminator (B0010-B0012)

References